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a Department of Respiratory Medicine, b Department of Virology/Immunology, c Auckland Healthcare Ltd,
Auckland, New Zealand
Correspondence to: Dr M L Wilsher, Respiratory Services, Green Lane Hospital, Auckland 3, New Zealand.
Received 22 December 1997; Returned to authors 25 February 1998; Revised version received 24 April 1998; Accepted for publication 2 June 1998
BACKGROUND
Although some studies have reported the
presence of Mycobacterium tuberculosis (MTb) DNA in
tissues affected by sarcoidosis, the data are conflicting. The aim of
this study was to collect prospectively tissue from patients with
sarcoidosis in whom tuberculosis had been excluded, and to use
polymerase chain reaction (PCR) to search for DNA sequences specific
for MTb.
METHODSFresh tissue samples (node or lung biopsy)
taken from 23 patients with newly diagnosed sarcoidosis, 10 with other
respiratory disease, and four patients with culture positive
tuberculosis were analysed using PCR to amplify a 123 bp fragment of
IS6110, the insertion element present in MTb, and nested PCR to further amplify an 85 bp sequence within the 123 bp product. DNA was also extracted from formalin fixed tissue from eight additional patients with sarcoidosis.
RESULTSMTb DNA was not detected in any of the
tissue samples from patients with sarcoidosis or other respiratory
disease but was found in all four patients with tuberculosis.
CONCLUSIONSThis study has shown the absence
of MTb DNA in lymph node and lung biopsy samples from patients with
sarcoidosis. MTb is therefore unlikely to be a factor in the
pathogenesis of this disease.
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