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Department of Child Health, University of Leicester
School of Medicine, Robert Kilpatrick Clinical Sciences Building,
Leicester Royal Infirmary, P O Box 65, Leicester LE2 7LX, UK
Correspondence to: Dr C O'Callaghan
Received 19 August 1999; Returned to authors 16 November 1999; Revised version received 12 January 2000; Accepted for publication 12 January 2000
BACKGROUND
The aim of this study was to determine
the relationship of the power and recovery stroke of respiratory cilia
using digital high speed video imaging. Beat frequency measurements
made using digital high speed video were also compared with those
obtained using the photomultiplier and modified photodiode techniques.
METHODCiliated epithelium was obtained by
brushing the inferior nasal turbinate of 20 healthy subjects. Ciliated
edges were observed by microscopy and the deviation of cilia during
their recovery stroke relative to the path travelled during their power
stroke was measured. Beat frequency measurements made by digital high speed video analysis were compared with those obtained using the photomultiplier and modified photodiode.
RESULTSCilia were found to beat with a forward
power stroke and a backward recovery stroke within the same plane. The
mean angular deviation of the cilia during the recovery stroke from the
plane of the forward power stroke was only 3.6°(95% CI 3.1 to 4.1). There was a significant difference in beat frequency measurement between the digital high speed video (13.2 Hz (95% CI 11.8 to 14.6))
and both photomultiplier (12.0 Hz (95% CI 10.8 to 13.1), p = 0.01)
and photodiode (11.2 Hz (95% CI 9.9 to 12.5), p<0.001) techniques.
The Bland-Altman limits of agreement for the digital high speed video
were -2.75 to 5.15 Hz with the photomultiplier and -2.30 to 6.06 Hz
with the photodiode.
CONCLUSIONRespiratory cilia beat forwards and
backwards within the same plane without a classical sideways recovery
sweep. Digital high speed video imaging allows both ciliary beat
frequency and beat pattern to be evaluated.
This article has been cited by other articles:
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